Convergent evolution of artemisinin and chloroquine resistance in Ethiopian Plasmodium falciparum parasites
, Engdaw, M, Alemayehu, DH, Deressa, JD, Adnew, B, Ayele, A, Keffale, M, Demisse, M, Seyoum, T, Shiferaw, M, Sime, H, Gebreegziabxier, A, Shimelash, A, Tafa, EB, Chali, W, Alemayehu, L, Kassa, FA, Zeleke, AJ, Hamza, TA, Tamirat, AB, Misganaw, T, Bogale, A, Abriham, ZY, Nasir, Y, Jebessa, G, Ejigu, LA, Yohannes, M, Kebede, BA, Ayele, MK, Abdo, M, Habtamu, G, Gizat, A, Phelan, J, Matlock, B, Fidock, DA, Campino, S, Assefa, A, Koepfli, C, Massebo, F and Tadesse, FG
(2025).
Convergent evolution of artemisinin and chloroquine resistance in Ethiopian Plasmodium falciparum parasites.
[Dataset].
Dryad.
https://doi.org/10.5061/dryad.1vhhmgr6c
The emergence of antimalarial drug resistance has threatened the control and elimination of malaria in Africa. Ethiopia, once a success story in case reduction, is now facing resurgence. This study examined the key drug resistance genes (Pfmdr1, Pfcrt, Pfk13, Pfdhfr, and Pfdhps) and mitochondrial genomes of 605 Plasmodium falciparum isolates from 15 districts across Ethiopia, varying in transmission intensity and P. vivax co-endemicity. A dominant PfMDR1 NFSND haplotype, associated with reduced lumefantrine susceptibility, was identified alongside near-fixation of the PfCRT CVIET chloroquine-resistant haplotype in specific areas. Concerningly, PfK13 variants associated with partial artemisinin resistance – R622I (10%), A675V (1.7%), and P441L (1.1%) – were expanding. Drug resistance markers were primarily found in settings with low transmission of P. falciparum and high levels of P. vivax coendemicity. Along with a distinct parasite lineage and limited gene flow, these findings suggest that local evolutionary and therapeutic pressures shape resistance. This underscores the urgent need for targeted genomic surveillance to guide tailored interventions and contain the spread of multidrug-resistant P. falciparum.
Keywords
Antimicrobial resistance, Genomics, Malaria, Biological sciences| Item Type | Dataset |
|---|---|
| Capture method | Experiment: Laboratory |
| Date | 16 July 2025 |
| Language(s) of written materials | English |
| Creator(s) |
Letebo, A, Vanheer, LN, Engdaw, M, Alemayehu, DH, Deressa, JD, Adnew, B, Ayele, A, Keffale, M, Demisse, M, Seyoum, T, Shiferaw, M, Sime, H, Gebreegziabxier, A, Shimelash, A, Tafa, EB, Chali, W, Alemayehu, L, Kassa, FA, Zeleke, AJ, Hamza, TA, Tamirat, AB, Misganaw, T, Bogale, A, Abriham, ZY, Nasir, Y, Jebessa, G, Ejigu, LA, Yohannes, M, Kebede, BA, Ayele, MK, Abdo, M, Habtamu, G, Gizat, A, Phelan, J, Matlock, B, Fidock, DA, Campino, S, Assefa, A, Koepfli, C, Massebo, F and Tadesse, FG
|
| LSHTM Faculty/Department | Faculty of Infectious and Tropical Diseases > Department of Infection Biology |
| Participating Institutions | Arba Minch University, Armauer Hansen Research Institute, London School of Hygiene & Tropical Medicine, London, United Kingdom, Radboud University Medical Center, Ethiopian Public Health Institute, University of Gondar, University of Werabe, Madda Walabu University, Woldia University, Dilla University, Addis Ababa University, Oak Ridge Institute for Science and Education, Columbia University Irving Medical Center, University of North Carolina at Chapel Hill, University of Notre Dame |
| Funders |
Project Funder Grant Number Funder URI |
| Date Deposited | 16 Jul 2025 13:35 |
| Last Modified | 17 Jul 2025 09:21 |
| Publisher | Dryad |
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